Coding

Part:BBa_K4940002:Design

Designed by: Kuang Minjie   Group: iGEM23_iZJU-China   (2023-10-10)


GlcDH-II-his


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 371
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since the gene has a clear sequence and has been proven useful based on many experiments, no certain considerations were in the design process. A his tag was fused to the protein for further purification.

Source

This gene was firstly extracted from the genome of Bacillus megaterium. Our team found the gene sequence from the NCBI database.

References